Introduction: Traditional dance training is designed to develop a well-balanced dancer, yet laterality has the ability to impair bilateral strength and functional symmetry. The aim of this study was to investigate the effects of lateral preference on elite level dancers by conducting a bilateral comparison of lower limb strength and pirouette performance. A pirouette is an essential turning skill in dance technique, wherein multiple revolutions are completed on a single supporting leg along the longitudinal axis. Methods: Eight highly trained female dancers performed three maximal effort single leg countermovement jumps (SCMJ) on each lower limb, as well as ten single pirouette en dehors in both the preferred and non-preferred directions. Results: A lateral preference questionnaire indicated a rightward bias was prevalent amongst this group of dancers for both footedness and preferred turning direction. Significantly more successful pirouettes were performed in the preferred (73%) compared to the non-preferred condition (50%). The SCMJ data demonstrated no significant differences in jump height and vertical ground reaction force (vGRF) at take-off, suggesting no bilateral strength asymmetry between the lower limbs. There were also no significant differences in any of the discrete biomechanical pirouette performance variables: displacement of the center of mass over center of pressure (COM-COP), trunk angle, knee flexion and external hip rotation of the gesture leg in retiré position, as well as GRF production and peak pelvic rotational angular velocity at push-off. However, non-significant trends toward greater COM-COP displacement at retiré (0.08 m ± 0.03 vs 0.06 m ± 0.04) and peak pelvic angular velocity at push-off (1.04 revs/s ± 0.20 vs 0.99 revs/s ± 0.22) were observed in the non-preferred compared to the preferred direction, respectively. Conclusion: Therefore, the lower success rate of non-preferred pirouettes may be a result of postural instability or the use of a less efficient weight transfer strategy.