To inform future intervention strategies and enhance professional esports players’ performance, this qualitative study investigated stressors, perceived stress responses, and coping strategies experienced by professional League of Legends players. Following criterion-based sampling, semi-structured interviews with 12 professional esports players were performed. The findings illustrate a variety of stressors related to team, performance, audience, and social media. Whereas players reported that perceived stress responses prior to competition (e.g., nervousness and excitement) seemed to be suppressed during competition, post-competition responses appeared to relate to the outcome of competition. Although a range of strategies were identified, players most frequently communicated with teammates or coaches and focused on performance when coping with stressors. Study results demonstrate a need to gain an in-depth understanding of stressors, coping strategies, and their effects on performance. In addition, it seems beneficial to teach players how to recognize and regulate perceived stress responses.