Challenge and threat responses have been seen to predict success in meaningful performance environments, however, it is not as clear whether challenge and threat states predict academic outcomes. We tested if predictions from the Theory of Challenge and Threat in Athletes (TCTSA) can be extended to an academic context, by considering antecedents and outcomes of challenge and threat states as well as whether cardiovascular markers predicted academic performance. Thirty-six undergraduate students were asked to give speeches on an academic topic, and their cardiovascular responses, overall annual marks, and marks in a specific presentation assessment were recorded. Challenge and threat indexes failed to predict either of the performance measures. Limited support was found for other hypothesised relationships of the TCTSA, with challenge states predicting greater reinvestment, which was opposite to that hypothesised. Results suggest that predictions of the TCTSA may not be as pertinent in understanding goal pursuit in academic settings.