New Zealand blackcurrant (NZBC) extract is a rich source of anthocyanins and in order to exert physiological effects, the anthocyanin-derived metabolites need to be bioavailable in vivo. We examined the plasma uptake of selected phenolic acids following NZBC extract supplementation alongside maintaining a habitual diet (i.e., not restricting habitual polyphenol intake). Twenty healthy volunteers (9 females, age: 28±7 years, height 1.73±0.09 m, body mass 73±11 kg) consumed a 300 mg NZBC extract capsule (CurraNZ™; anthocyanin content 105 mg) following an overnight fast. Venous blood samples were taken pre and 1, 1.5, 2, 3, 4, 5, and 6 h post-ingestion of the capsule. Plasma concentrations of vanillic acid (VA), gallic acid (GA), and protocatechuic acid (PCA) were analysed by reversed-phase high-performance liquid chromatography (HPLC) and the HPLC analysis identified two dihydroxybenzoic (VA and PCA) and one trihydroxybenzoic acids (GA) in plasma following NZBC extract supplementation. Habitual anthocyanin intake was 168 (95%CI:68–404) mg⋅day-1 and no associations were observed between this and VA, PCA, and GA plasma uptake by the NZBC extract intake. Plasma time-concentration curves revealed that GA, and PCA were most abundant at 4, and 1.5 h post-ingestion, representing a 261% and 320% increase above baseline, respectively, with VA remaining unchanged. This is the first study to demonstrate that an NZBC extract supplement increases the plasma uptake of phenolic acids GA, and PCA even when a habitual diet is followed in the days preceding the experimental trial, although inter-individual variability is apparent.