The aim of this study was to quantify whole and peak physical characteristics of Under (U)14 and U16 elite youth female soccer, and compare by position and age-group. Data was collected using 10Hz GPS units from 431 match observations, during 50 matches involving 201 players (U14 n=93; U16 n=108) representing Regional Talent Centres in The Football Association’s Girl’s England Talent Pathway League. Whole match data were reported as absolute and relative; total (TD), high speed running (HSR; ≥3.46m·s-1), very high speed running (VHSR; ≥ 5.29 m·s-1), and sprinting (SPR; ≥6.26 m·s-1) distance, and maximum velocity. Moving average analysis determined peak data for 1-10 minute durations. Linear mixed modelling and effect sizes (ES) established position-specific differences. Results showed U16s covered greater; absolute distance at all speeds (small-moderate ESs; p<0.001); relative VHSR and SPR m·min-1 (small-moderate ESs; p<0.001); peak TD and HSR m·min-1 (small ESs) across several peak-durations, and VHSR m·min-1 (small ESs; p<0.001) across all peak-durations compared to U14s. Position-specific differences were observed across all positions between and within both age-groups, identifying whole and peak physical characteristics are age- and position-dependent within elite youth female soccer match-play. Findings may facilitate informed coaching practices and training programme design, talent identification and development processes.