Key assessments predominantly used to assess soccer players are; maximal aerobic capacity, power and strength, sprint speed, change of direction and agility. The key physical characteristics of soccer players are predominantly lower-body focused with great emphasis on the knee extensors (quadriceps) and knee flexors (hamstrings), when compared known at the hamstring:quadricep ratio. The various different physical characteristics require variations of the lower body working systematically to produce the required movements. Physical performance characteristics have been shown to have various relationship with strength as a the main focus.The aim of the this study is to determine the greatest predictive performance characteristics to calculate the functional hamstring: quadricep ratio (H:Qfunc) 31 female soccer players, descriptive statistics represented as mean±SD, age, 18.65± 1.89 years; height, 165.48 ± 8.73 cm; mass, 62.72 ± 8.51 kg. Performance tests included, 10 m sprint, 30 m sprint, 505 change of direction, isometric mid-thigh pull (IMTP), countermovement jump (CMJ) and isokinetic strength of the knee. Participants performed three trials per performance measure and data collected for further statistical analysis. H:Qfunc ratio was generated by flexion extension of the knee using isokinetic dynamometer and ratio was determined using hamstring eccentric peak torque divided by concentric quadricep peak torque measured at 60degrees/sec Dominant leg was determined as the leg with the greatest H:Qfunc. Pearson’s Correlation, linear regression and independent samples t-test were conducted for statistical analysis. A significant (p<0.05) linear regression model explained 49.1% of variance to predict H:Qfunc using COD deficit, 30 m sprint speed, IMTP peak force (PF) and CMJ PF. Age differences were compared and revealed significant differences between University and College players in Age ( t29 = 7.781, p = 0.000, p<0.001), COD H:Q (t29 = -2.546, p = 0.017, p <0.05), COD Fastest t29 = -3.160, p = 0.004, p <0.05) COD Deficit (t29 = -3.191, p = 0.003, p <0.05), H:Q Functional (t29 = 3.215 p = 0.003 p <0.05) and H:Q Combined (t29 = 2.837, p = 0.008, p <0.05). The results of this study identify a regression model which can predict H:Qfunc using soccer specific performance characteristics. Age differences between characteristics were also prevalent suggesting University level players had greater H:Qfunc and quicker COD ability enhancing the importance of hamstring strength focus for performance benefits in movements that require increased load of eccentric hamstring activation.