Daily intake of anthocyanin-rich New Zealand blackcurrant (NZBC) extract can enhance exercise-induced fat oxidation. It is not known whether habitual dietary anthocyanin intake and body composition affects blackcurrant-induced fat oxidation or if daily intake is required. We examined effects of daily and every-other-day intake of NZBC extract on metabolic and physiological responses during moderate-intensity walking. Sixteen physically active males (age: 24±6 yr, body mass: 78±16 kg, BMI: 24.7±4.2 kg·m-2, body fat: 15.2±5.0%) volunteered. A randomised, cross-over design with a control condition was used and habitual dietary anthocyanin intake quantified. For intake conditions, participants consumed 2 capsules of NZBC extract (210 mg of anthocyanins) daily or every-other-day for 14 days (14-D and 14-EOD) with 14-days washout. Final two capsules were taken 2-hr before the walk (speed: 5.7±0.7 km·hr-1). There was a trend for lower respiratory exchange ratio and carbohydrate oxidation with changes only for 14-D. Fat oxidation was increased only for 14-D (P<0.05) with 50% of the participants more than a 10% change. In 14-D, there was a positive correlation for BMI and body fat % with the absolute change in fat oxidation but not with dietary anthocyanin intake. Daily intake of NZBC extract is required to enhance exercise-induced fat oxidation. Enhanced exercise-induced fat oxidation by daily intake of NZBC extract is related to body composition but not to habitual dietary anthocyanin intake in physically active males. Daily anthocyanin intake seems to allow the gradual build-up and maintenance of anthocyanin-derived metabolites required to alter mechanisms for exercise-induced substrate oxidation.