The American College of Sports Medicine (ACSM) endorses mirror use in exercise settings to promote performance feedback and therefore, the influence of mirrors on affective responses requires exploration. This study aimed to investigate the impact of self-awareness (mirror use) on female affective responses during an aerobic exercise session. Twenty-five female undergraduates took part in two (mirrored; non-mirrored) 45-minute aerobic exercise sessions. Affective responses, measured through the Felt Arousal (FAS; Svebak & Murgatroyd, 1985) and Feeling Scale (FS; Hardy & Rejeski, 1989), were examined at four time points. Significant interactions between condition and time for both felt (F(2.155, 51.726)=9.275, ηp2=0.279, p≤0.001) and feeling (F(3, 72)=7.285, ηp2=0.233, p≤0.001) scores were observed. There was not consistent evidence to show significant differences in affective responses between a mirrored and non-mirrored environment shown through post-hoc analysis. Self-esteem and objectified body consciousness (OBC) were not related to affective responses. The negative feelings reported by females in the funnelled debrief did not reflect in the affective responses measured. Therefore, future research could employ alternative measures to explore the impact of self-awareness on affective responses in exercise settings.