Intake of the catechin epigallocatechin gallate and caffeine has been shown to enhance exercise-induced fat oxidation. Matcha green tea powder contains catechins and caffeine and is consumed as a drink. We examined the effect of Matcha green tea drinks on metabolic, physiological and perceived intensity responses during brisk walking. Thirteen females (age: 27±8 yr, body mass: 65±7 kg, height: 166±6 cm) volunteered. Resting metabolic equivalent (1-MET) was measured using Douglas bags (1-MET: 3.4±0.3 ml·kg-1·min-1). Participants completed an incremental walking protocol to establish the relationship between walking speed and oxygen uptake and individualize the walking speed at 5- or 6-MET. A randomized cross-over design was used with participants tested between day 9 and 11 of the menstrual cycle (follicular phase). Participants consumed 3 drinks (each drink made with 1 gram of Matcha premium grade, OMGTea Ltd UK) the day before, and 1 drink 2 hours before the 30-min walk at 5- (n=10) or 6-METs (walking speed: 5.8±0.4 km·h-1) with responses measured at 8-10, 18-20 and 28-30 min. Matcha had no effect on physiological and perceived intensity responses. Matcha resulted in lower respiratory exchange ratio (control: 0.84±0.04; Matcha: 0.82±0.04) (P < 0.01) and enhanced fat oxidation during a 30-min brisk walk (control: 0.31±0.10; Matcha: 0.35±0.11 g·min-1) (P < 0.01). Matcha green tea drinking can enhance exercise-induced fat oxidation in females. However, when regular brisk walking with 30-min bouts is being undertaken as part of a weight loss program, the metabolic effects of Matcha should not be overstated.